![]() ![]() The proteins bind to the membrane through hydrophobic and electrostatic interactions. The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The nitrocellulose membrane (0.2 µm pore size) in the stack is composed of 100% pure nitrocellulose to provide high-quality transfer. This membrane has lower background levels and increased sensitivity for fluorescent probing than regular PVDF membranes. The proteins bind to the membrane through hydrophobic interactions. The membrane is compatible with commonly used detection methods such as staining, immunodetection, and fluorescence. The PVDF membrane (0.45 µm pore size, low fluorescence) has higher binding capacity than nitrocellulose. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins. The Power Blotter Select Transfer Stacks are pre-assembled stacks that come with an integrated preactivated PVDF or nitrocellulose transfer membrane for dry blotting of proteins from polyacrylamide gels. Images captured using automatic exposure on an iBright FL1000 Imaging System. Proteins were transferred in 7 minutes using a Power Blotter Select Transfer Stack (left, PB5310 PB3310) and Power Blotter Pre-cut Membranes and Filter stack (right, PB9320 PB7320), probed with target-specific primary antibodies and fluorescently conjugated secondary antibodies. Western blot analysis of several targets (KLH, EGFR, Hsp90, PDI and Cyclophilin B protein) was performed by loading serially diluted HeLa cell lysate with KLH spike (starting at 7.5 µg HeLa lysate, 7.5 µg KLH spike per well, serially diluted 2:3) onto Bolt 4-12% Bis-Tris Plus gels. ![]() Power Blotter Select Transfer Stacks and Power Blotter Pre-Cut Membrane and Filter stacks efficiently transfer high, mid, and low molecular weight proteins. Power Blotter with Pre-cut Membranes and Filters and Power Blotter 1-Step Transfer Buffer Porous Cellophane (20 x 35.Power Blotter with Select Transfer Stacks.Porous Cellophane (20 x 35.5 cm), 50 sheets.Hinged Lid with Stainless Steel Cathode.Molded Base with Platinum-Coated Titanium Anode and High Voltage Leads.Minimal buffer requirements-reagent cost and preparation time are reduced.Vented electrodes-prevent buildup of bubbles which may impair transfer.Durable iridium oxide and stainless steel electrodes-allow for contamination free, consistent transfer.Requires minimal current-does not generate the excessive heat that can dry out the transfer stack and halt transfer or damage transfer units.Included safety circuit breaker-limits voltage and current from the user's power supply, preventing electrical damage to the transfer unit.The TE77X has a 21 x 26 cm transfer area for a capacity of up to 12 mini gels at one time. The TE70X has a 14 x 16 cm maximum transfer area which can handle up to four mini gels using the stacked format. ![]() They support as many as two layers of gels being transferred simultaneously. The TE70X and TE77X Semi-Dry Transfer Units are efficiently designed to use minimal buffer to transfer proteins from polyacrylamide gels in less than an hour. ![]()
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